Here is kortingscode fiets en wandelbeurs my protocol.
Place gel into electrophoresis unit.
Carefully pour the agarose into the gel tray until it is approximately 1/3 of the way up the teeth of the comb.
Keep warm if the class will use it within a half hour.The gel will be removed from the box for you to chocolate makes me sleepy record your observations on the activity sheet.The size of the pores in the gel and the size of the fragment trying to move will determine the rate at which each fragment progresses.After the lab, the TBE buffer solution may be poured back into the container and reused for the Restriction Digest Laboratory.To prepare a construct for targeting of a specific mouse locus, see.Which dye likely contained the smallest molecule?Purify the transgene fragment using one of the following methods: Electroelution followed by purification over a deae minicolumn; (Schleicher Schuell Elutip minicolumn, catalog #27370).Please rinse and air dry both the gel trays and gel boxes in distilled water.Gel Disposal: When lab is complete, collect all gels in the plastic bags and dispose of in trash.A micropipet is a very delicate, expensive instrument that is used to dispense an extremely precise and very small volume.
To aspirate (pull in) the liquid, first depress the plunger to the first stop, place vertically into the liquid to a depth of about 2-3 mm and slowly release the plunger until it returns to its up position.Alison Hayward and Aurora Burds Connor, Jan 2007.For Orange G use 100 mg of dye and make up as above.The power supply may continue to produce some voltage even when the power has been turned off.Often the numbers before the decimal place will be in a different color to those that come after.Determine the components of an unknown dye mixture.We know this because they move towards the positive electrode.An additional 5 ml of mineral oil is added and again vortexed for 15 seconds, creating more microdroplets and also diluting them to reduce their coalescence.Inspect all power cords, patch cords, banana jacks and plugs for any defects, such as cracked and dried-out insulation and loose or wobbly banana jacks or plugs.Let the gel harden without disturbing it for about 10 minutes.
For more information on the differences between these types of mice, please see.
You may be sharing dye samples and the agarose gel with another lab group.